Protein Extraction

His-tag Protein Purification Mini Kit

SMART™ Bacterial Protein Extraction Solution

● Description Simple total Protein extraction from Bacteria without Sonication step §  Protein extraction within 25 min from G(-)/G(+) Bacteria §  Cytosolic protein and Inclusion body isolation from recombinant strain §   Higher efficiency than Sonication method. §  Non-ionic detergent provides a gentle and efficient method §  Protein stability preservation effect by Protein stabilization buffer   SMARTTM Bacterial Protein Extraction Solution is designed for fast and easy extraction of total proteins from bacteria without sonication or precipitation step. This solution utilizes a proprietary non-ionic detergent and buffering system, so, it is effective for protein analysis and extraction with gram positive and negative bacteria. In addition, the SMART™ Bacterial protein extraction solution offers several folding increase in the yield of soluble protein(Refer to Fig1.). Extracted Protein can be stable because there is no repeated freezing and melting step. Therefore, SMARTTM Bacterial Protein Extraction Solution is optimal reagent for recombinant protein work for routine bacteria samples.

PRO-PREP™ Protein Extraction Solution (C/T)  ● Description High purified total protein extraction kit for cell or tissue. §  Simply extract protein from all kinds of cells and tissues §  Monomeric  formation is made by ionic detergent.     Effective protein separation is possible for western blot analysis     Efficient protein molecular weight measurement §  Absorbance hindrance is not contained to eliminate error rate. §  Protein stabilization buffer is included for protein stabilizing. §  5 kinds of protease inhibitor included to minimize protein degradation. §  Only takes 20~30min for protein extraction   PRO-PREP™ Protein Extraction Solution can be simply extract protein from all kinds of cells and tissues. The kit contains 5kinds of protease inhibitors so it is possible to extract very highly purified proteins. Usually detergent used in protein extraction consists of both hydrophobic tails and amphiphilic molecule and hydrophilic head. The two parts are joined to form a micelle. It is solubilized protein forming a lipid detergent mixed micelle andtransmembrane protein forming a protein lipid detergent complex. The extent of micelle formation is termed as CMC(critical micelle concentration). Which is important for high efficiency as well as high purity of protein extraction. CMC is influenced by pH, temperature, ionic strength, multivalent ions of organic solvents, purity of detergent and so on. Depending on ionic charateristics, a detergent can be categorized as ionic detergent, non-ionic detergent, and Zwitterionic detergent. Ionic detergent can be further classified into either cationic detergents. These are highly denaturant which have a specific property to isolate protein as a monomeric form and so often used in western blot analysis and measurement of molecular weight. Non ionic detergent such as Triton X-100 are less protein denaturant and often employed in protein interaction. Zwitterionic detergent such as CHAPS have both negative and positive charge head at the same time, more effective in protein-protein interaction than non ionic detergent and its extxtent of protein denaturation is less than that of ionic detergent. It is very important to select the optimal buffer and detergent for protein extraction.

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Protein Synthesis

YLEX Yeast Expression System (Yarrowia lipolytica) 


 F-Detector™ Cell Free Labeling Kit 

● Description Cell free labeling product to extract protein after in vitro protein expression by fluorescein. §  Non-isotope labeling §  Protein analysis at real-time. §  Offering the sensitivity over radioactive isotope using fluorescent imaging instrument. §  No decreasing bioactivity by Labeling protein. §  Direct detection through UV trans-illuminator §  Detection of proper CE by Laser induced fluorescence.    : It is possible to cooperate with Gene function analysis and drug screening and mutation      screening etc. F-DetectorTM Cell Free Labeling Kit serves a sensitive, non-isotopic, fluorescence-based method for the detection of nascent proteins directly in gels with high signal-to noise ratios after in vitro synthesis. A fluorescent technique using F-Detector™ Kit offers a simplified alternative method that dramatically reduces the time required to obtain data due to the rapid detection of the protein bands immediately after electrophoresis without any downstream processing and eliminates manipulations associated with radioactivity( i.e. gel fixing and drying or transferring to a membrane). It also eliminates the requirement for electroblotting associated with other non-isotopic technologies based on the biotin moiety.

Genelator™ in vitro Transcription/Translation Kit ● Description Easy and fast in vitro Transcription/Translation in one tube without cell culture. §  Fast protein production within one hour. §  High yield protein harvest, amount of 300 ~ 500 mg/ml. §  Wide range application from small peptide to protein. §  Simple scale-up or scale-down. §  Easy target protein isolation through Western blotting, fluorescent gel scanning or     autoradiography.     (Non-radioactive labeling system: F-Detector) §  Suitable for aggregation-prone protein production, resulting from Eukaryote. §  Various gene expression in one tube. §  T7 RNA polymerase added in advance.   Excellent solubility of protein for Origin of Eukaryote gene expression. (active protein production) The GenelatorTM in vitro Transcription/Translation Kit uses highly productive E. coli lysates, which contain all transcriptional and translational machinery components (chaperones, ribosomes, ribosomal factors, tRNAs, aminoacyl-tRNA synthesis, etc.) as well as T7 RNA polymerase. It is a coupled transcription- translation system that can be used to express full-length proteins from T7 or E.coli promoters in a single-step reaction using supercoiled (plasmid) DNA templates. Using the Genelator™ Kit, up to 600ug/ml biologically active protein can be synthesized within 60minutes. The synthesis reaction can be easily scaled up (to produce mg amount of protein synthesized increases linearly with increased reaction volume).  The GenelatorTM in vitro Transcription/Translation Kit  is more suitable for the enhanced soluble production of aggregation-prone protein compared to conventional cell-free protein synthesis. Genelator™ Kit is prepared from genetically engineered E.coli strains which are modified to have enhanced levels of folding-related factors as a folding aid for better solubility of proteins. Genelator™ Kit serves as a powerful technology for the production of aggregation-sensitive proteins originated from eukaryotes.  We expect this Genelator™ Kit will be helpful in production of a higher level of soluble proteins for further biochemical analysis and proteomics research such as structural genomics and functional genomics. And it is applicable to preparation of protein samples required in the actively growing research areas such as structural genomics, functional genomics, protein chip, diagnosis, protein engineering, bioelectronics including biosensor, and lab-on-a-chip.

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Event

Title	[NEW] Gods’ Choice (June) “Guardians” (Artemis & Viral Gene-spin™)
Event period	2013-06-20 ~ 2013-06-20	File

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Q & A

No	Title	Date	State
2	Viral Gene-spin	12-04-10 18:32	Answer is completed
1	Redsafe & Sequencing	12-04-06 10:55	Answer is completed

Liên hệ :http://www.amb.vn

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Delivery

How To Order Orders can be made using any of the following methods: Email your order to clicking here Fax your order to (04) - 35627 747 or your distributor Phone your order to (04) - 35627 763 To place your order, the following information should be provided; Your name, telephone and fax number Customer account number Purchase order number Invoice address including department (both Ship to and Bill to) DHL, FedEx or TNT account number(if available)	Download our complete catalogue
Price Information To inquire about prices, please phone +82-31-778-7807 or email: amb.vn
Technical Support If you need a technical support, please email me at tech@intronbio.com
International Ordering Information For International Customers, please contact the iNtRON Distributor within your country. If your country does not have an authorized distributor, contact us with your questions or to place your order. Your name, telephone number, and e-mail address Purchase order number Name, telephone number, and e-mail address of the person using the product Shipping address Billing address Catalog number, name, size, and quantity of each item ordered
Quality Guaranteed We stand behind our products. If you are not satisfied that our products are of the highest quality available, we  will replace or refund your money. Our products and systems are certified by ISO 9001 / ISO 14001 Standard in 2004.
Large Quantities or Special Packaging iNtRON understands the needs of large quantity users for the highest quality enzymes and reagents with exc eptional lot-to-lot reliability and performance, delivered on time and as specified. We have years of experience  in supplying cost-effective enzymes in bulk (up to billions of units) for OEM, R&D, bioprocess scaleup,  and manufacturing of biopharmaceuticals, and for use as components in clinical diagnostics. Custom or  contract manufacturing of non-catalog enzymes is also possible, using iNtRON's extensive staff experience  and proprietary methodologies in fermentation, cloning, mutagenesis, expression, and protein purification. Take advantage of our expertise, experience, and proven commitment to product quality and customer satisfaction.  Contact our staffs in confidence to see how we can help you: Telephone: +82-31-778-7807 Fax: +82-31-739-5072
New Country Discount If you are setting up a new Country Distributing strategy in any territory, not for iNtRON Distributor territory,  we would like to help. Please contact our Overseas Department for details and a quote number entitling you to  a special discount on all catalog products and sizes for six months.
Pricing and Terms We stand behind our products. If you are not satisfied that our products are of the highest quality available,  we will replace or refund your money. Our products and systems are certified by ISO 9001 / ISO 14001  Standard in 2004.
Environmentally Conscious Packaging Most iNtRON products are shipped on reusable frozen gel packs in the smallest polystyrene foam  container possible. Polystyrene can be recycled. For more information on our packaging, please contact  Overseas Department.
Contact Information

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Buffer & Column

RNA Series Column + Collection tube 

● Description It is spin column which is included in the RNA extraction product line. CAPS (Blister packing system) is applied to minimize contamination(Fig 1). This spin column is used to purify RNA at the lysis step and binding step etc. 2.0ml collection tubes are also included in the product package.

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Total RNA Prep

R&A-BLUE™ Total RNA Extraction Kit

● Description Solution type product which can extract high yield and purity of Total RNA from cell, tissue or plant, etc.  and possible to store at Room Temperature. § Can be stored at room temperature for 1 year, and at 2-8°C for 2 years by oxidation control of      Phenol. § Excellent stability and easy to use with mild smell by no formation of ortho-chlorophenol. § Effectively control of gDNA inflow, which affect product oxidation in long-term storage, thru    antioxidant activity. § Excellent efficiency of phase separation on both small amount of samples and large amount of    samples. § Maximized yield by high dissociation-efficiency of solid tissue and cell wall.  Possible to extract RNA effectively from the sample which has metabolite like lipid and  polysaccharide.    R&A-BLUE™ Total RNA Extraction Kit is exemplary for quick, economical, and  efficient isolation of total  RNA or the isolation of RNA from samples of human, animal, plant, bacterial and viral origin. A reagent of  phenol and guanidine-salt inhibits RNase activity, and so the reagent maintains integrity of the RNA during  sample homogenization or lysis. A biological sample is homogenized or lysed in R&A-BLUE™ and the  homogenate is separated  into aqueous and organic phases by chloroform addition and centrifugation. RNA  remains exclusively in the aqueous phase, DNA in the inter phase and proteins in the organic phase. RNA is precipitated from the  aqueous phase by addition of 2-propanol, washed with ethanol and solubilized. R&A-BLUE™ Total RNA Extraction Kit performs well with large or small amount of tissue or cells and many samples can be simultaneously extracted. This is one of the most effective methods for isolating total RNA and  can be completed in only 1 hour starting with fresh tissue or cells. The procedure is very effective for isolating  RNA molecules of all types. The isolated RNA is intact with little or no contaminating genomic DNA. This RNA  can be used for Northern blot, cDNAsynthesis, in vitro translation, cloning and RT-PCR.

 easy-spin™ Total RNA Extraction Kit 

● Description Spin type product which can extract high purity and yield total RNA from cell and tissue in easy and fast way without genomic DNA contamination §  The combination solution type and spin type §  Minimum of genomic DNA contamination §  Extraction of high yield and purity total RNA §  No extra alcohol precipitation, column use for obtaining of total RNA §  High reproducibility in the result by using column §  Extraction of RNA within 30 minutes easy-spinTM (DNA free) Total RNA Extraction Kit is designed to extract total RNA from cell and tissue in easy and fast way without mixing of genomic DNA. RNA extraction product is normally  divided into column type and solution type. Solution type(phenol use) has higher yield comparing to spin type and also can extract large amount of RNA. Even though alcohol precipitation step is required due to the use of phenol, solution type can economically extract RNA so that is common method for RNA extraction. For spin type, no phenol is required and pure RNA can be extracted without alcohol precipitation.  This can help researcher to extract RNA from a lot of samples in a short time  with guarantee of the safety as well as convenience of the test.  Solution type using phenol has a lot of experimental error at times  and spin type rarely has the error  so the reproducibility of test is approved but genomic DNA is often mixed.  easy-spinTM (DNA free) Total RNA Extraction Kit was developed to have the advantages of the both solution type and column type, alcohol precipitation is not required but extraction of total RNA is done within  30 minutes without mixing of gDNA. To minimize contamination and use the RNA for pathogen research, CAPS (clean automatic packaging system) is applied to columns(Table 1) .

IQeasy™ Plus Blood RNA Extraction Mini Kit 

● Description Spin type product with 2 columns which can extract high yield and purity total RNA from whole blood sample without mixing of gDNA. § Effective extraction of total RNA from whole blood sample § gDNA remover spin column system applied    The fast removal of gDNA by 2 column system    Minimized mixing of gDNA without extra DNase treatment § Extraction or purification of high yield and purity RNA within 30 minutes § Extraction of High purity RNA based on non-phenol method     IQeasy™  Plus Blood RNA Extraction Mini Kit  is designed to extract high purity RNA from whole blood sample within a short time. RNA extracted from whole blood can be easily exposed to many contaminant and damaged by intracellular and extracellular RNase. To minimize those problem, the lysis buffer of our kit is optimally designed to deactivate endogenous nuclease (RNase A) which  is released from ruined cell when the lysis of a white blood is done. In addition, the kit based on our 2 column method, gDNA remover column for the removal of gDNA and RNA binding filter column including high silica content, can effectively extract high purity and yield total RNA. The high purity RNA extracted by IQeasy™  Plus Blood RNA Extraction Mini Kit based on non phenol method can be used for real-time PCR and microarray, etc.

easy-spin™ llp Plant RNA Extraction Kit (Mini-prep)  ● Description Spin type product which can extract  total RNA of mini scale from various plants §  Easy and simple extraction of total RNA from various plant tissue including leaf, trunk, root     and seed, etc.     Extraction of total RNA from  seed containing a lot of reserve materials    §  Minimum of  factors of contamination in plant tissue §  Minimum of contamination of genomic DNA §  Extraction of pure RNA with no alcohol precipitation step §  Guarantee of high purity and yield   easy-spin™ IIp Plant RNA Extraction Kit(Mini-prep) is designed to extract total RNA of mini scale from various plant tissue.  This product works using easy -BLUETM Solution, phenol but extra alcohol precipitation is not required and total RNA can be extracted in easy and convenient way by using silica membrane. Extra pre lysis buffer  is available  for  pre lysis of sample at the beginning and  the added function for precipitation of reserve material in plant to prevent the low purity of total RNA by  contaminants  such as polyphenolic compound or polysaccharide . IQeasy™ Plus Plant RNA Extraction Mini Kit ● Description  Spin type product with 2 column which can extract total RNA from plant such as various leaf,    trunk, root and seed § Extraction of total RNA from various plant sample    Effective extraction of total RNA from seed containing a lot of metabolites § Extraction of high purity and yield RNA    The fast removal of gDNA and extraction of RNA within 20 minutes by 2 column system    The removal of more than 99% of gDNA without extra DNase treatment         §  RNA extraction based on non-phenol method IQeasyTM Plus Plant RNA Extraction Mini Kit is designed to extract total RNA from leaf, trunk, root and seed of various plants. Plant accumulates variety of metabolites through photosynthesis and those metabolites would  be an inhibitor which has an effect on extraction of nucleic acid. To remove this inhibitor, high concentrationchaotrophic salt or phenol should be used under lysis step. However, for  rice, barley, wheat and seed containing a lot of starch or bean, green gram, pea and seed containing a lot of protein as well as soil, if alcohol is added for binding, too much precipitation  occurs and also the nucleic acid is precipitated (co-precipitation), that brings the sample including less RNA. To overcome this problem, IQeasyTM Plus Plant RNA Extraction Mini Kit was developed. IQeasyTM Plus Plant RNA Extraction Mini Kit works effectively on the plant containing a lot of metabolites  for extraction of total RNA and the high yield and purity RNA can be extracted because chaotrophic salt is not used and inhibitor,  the factor of obstruction is removed under lysis step. 2 column system is used, one is a sieve spin column which can remove the supernatant fast in homogenate and another is a gDNA binding column for removal of gDNA. As mentioned earlier, the extraction of RNA from various plant can be done within 20 minutes because of the absence of enzymatic lysis.  Two kinds of protocol are provided for both tissue and species  for easy and simple extraction of high purity and yield total RNA.    IQeasy™ Plus CTB RNA Extraction Kit  ● Description Spin type product with 2 columns which can effectively extract the high purity and yield total RNA from cells, tissue, bacteria (gram+/-) and yeast. § Effective extraction of total RNA from various samples including cells, tissues, bacteria   (gram+/-). § Specialized protocol for each sample (total three types) § gDNA remover spin column system applied    The fast removal of gDNA by 2 column system § Minimized mixing of gDNA without extra DNase treatment § Extraction or purification of high yield and purity RNA within 30 minutes § Extraction of High purity RNA based on non-phenol method   IQeasy™ Plus CTB RNA Extraction Mini Kit is designed to extract high purity total RNA from cells, tissue, gram(+/-) bacteria and yeast sample within 20 minutes. Without no extra enzyme treatment, RNA extraction is available with buffer RPL (pre-lysis buffer) only. In order to minimize the mixing of gDNA, gDNA Remover Spin Column is provided but no DNase treatment is required, that helps extraction of high purity total RNA which can be used for microarray. RNA binding filter column including high silica content is also provided for the effective extraction of high purity and yield total RNA. In addition, the specialized protocol for each sample helps easy extraction as well as obtaining of highly reliable and reproducible total RNA(Table 1.) This kit is based on non-phenol method and specialized for the high purity RNA which  can be used for real-time PCR and microarray, etc.

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