plasmid & probe DNA

DNA-midi™ GT Plasmid DNA Purification Kit 

● Description

 DNA-midi GT plasmid DNA Purification Kit is designed for High purity of DNA purification by  Gravity

 flow.

· Efficient DNA purification is available for Midi Scale samples.

   High copy number: up to 60 ml culture volume

   Low copy number: up to 120 ml culture volume

· Equality to that obtained by 2x CsCl-gradient centrifugation.

· Eliminates the use of phenol, chloroform, ethidium bromide, and cesium chloride, minimizing exposure

 to, and disposal of hazardous materials.

· Time saving: Complete the process in less than 120 minutes by gravity flow.

DNA-Midi™ GT Plasmid DNA Purification Kit is designed for efficient purification of high quality plasmid 

DNA from bacterial cells. This kit provides alkaline lysis reagents and columns packed with anion-exchanger resins.

The entire midi prep process can be completed within 120 minutes through gravity-flow procedure and up to 

100 ㎍ of plasmid DNA can be expected. The plasmid DNA is free from protein, genomic DNA and RNA

 contaminants. This pure plasmid DNA is suitable for downstream applications such as transfection, in

 vitro transcription and translation, and all enzymatic modifications.

● Applications

· Ligation & Transformation

· Transfection

· PCR & Real-time PCR

· Automated fluorescent sequencing

· Microinjection

    · Restriction digestion etc.

DNA-maxi™ SV Plasmid DNA Purification Kit 






DNA-midi™ SV Plasmid DNA Purification Kit 

● Description DNA-midi SV Kit is designed for easy and fast high yield midi scale, plasmid DNA isolation and purification.  · Efficient isolation and purification is available for Midi Scale.    High copy number : 30~50 ml culture volume.     Low copy number : 100 ml culture volume.  · Takes 30 minutes to extract high yield plasmid DNA (vacuum method).  · Pre-filtration column applied to remove Cell lysate.  · Centrifugation method(Swing bucket rotor only) makes Ethanol settlement needlessness.  · Simple and stable plasmid DNA isolation and purification with over 30 Kb.  · Lysis & Neutralization indicator (Lysis Viewer) applied. DNA-midi™ SV Plasmid DNA Purification Kit is designed to extract high purity and concentrate plasmid  DNA from midi scale bacterial cell culture volume using silica  membrane and optimized alkali lysis buffering  system. Two column system helps easy isolation and purification with pre-filtration column and DNA  binding membrane applied binding membrane. So you can extract and purify DNA within 30min using the  vacuum method (Fig 1.  Combination of Pre & Binding Column). Neutralization buffering system affects pH  neutralization and binding for stable extraction and purification from over 30Kb  size plasmid DNA. Effective  chromosomal DNA, cell debris removal  is also possible with that system. Lysis Viewer is applied to visual  pH confirmation as lysis& neutralization indicator, so you can easily find the pH condition during the  alkaline lysis step. High-copy plasmid DNA and low-copy plasmid DNA are all available to use.  Washing Buffer A is separately offered for efficient isolation and purification of plasmid DNA from endA+  strain(HB101, the JM strains, NM series strains, PR series strains). ● Applications · Ligation & transformation · PCR & Real-time PCR · Automated fluorescent sequencing · Transfection · Restriction digestion  etc.      MEGAquick-spin™ Total Fragment DNA Purification Kit

● Description

PCR Product, agarose gel DNA, DNA clean-up own functional spin type product.

 · PCR Product and agarose gel DNA isolation and purification by one kit.

   DNA size : 60 bp ~ 20 kb length

   Recovery of PCR Product >  80 ~ 95 %

   Recovery of Agarose Gel DNA > 60 ~ 90 %

 · No shearing and stable purification is possible with 20Kb long fragment.

 · Short fragment bypassing can intersection by column for under 100bp short fragment.

 · Easily find pH changes by pH indicator. Increased lysis capacity induce gel lysis for

   simple DNA recovery.

MEGAquick-spin™ Total Fragment DNA Purification Kit is designed to extract DNA from both PCR product and 

agarose gel. BNL Buffer include chaotropic salt to maximize the binding effect on silica membrane so that, 60 bp to 20 

Kb size isolation and purification is possible. It is designed to extract and purify long DNA over 20kb without shearing. 

Most effective removal of primerdimer is implemented by this kit to make a efficient DNA purification. pH indicator 

is added in BNL Buffer(Lysis Buffer) to find the pH changes for optimized pH confirmation.

● Applications

· Ligation & transformation

· Ligation & Transformation

· PCR

· Labeling

· Restriction digestion

· Microarray analysis

· in vitro transcription etc.     

PROBER™ Probe DNA Purifying System

● Description

Effect of charging condition by Advanced Sephadex Resin makes yield maximized. Only two times of spin down can purifylabeled probe DNA.

· Maximized yield by advanced Sephadex Resin

    Efficient for small quantity labeled probe DNA

    Optimized for small size(100 bp ~ 1 Kb) labeled probe DNA purification

    Effective removal of dNTP, free isotope and chemicals after Probe DNA labeling 

· Only two times of Spin down can complete purification

PROBER™ Probe DNA Purifying System is disposable column, which is designed for un-incorporated nucleotide removal

 after DNA labeling during react of polymerization or end-labeling, random primer labeling, nick translation using sephadex.

Sephadex pillar is build up by the spin down using charged sephadex resin. You can get labeled probe DNA if load 

the sample in thesephadex pillar and perform spin down. Especially, our advanced sephadex resin makes effective 

removal of dNTP, free isotope, enzyme after probe DNA labeling step by chemical method or isotope. Two times 

of spin down step is  enough for simple and high probe DNA reproducibility.

● Applications

· Purification after random primer labeling

· Purification after Nick translation  or polymerization

· Purification after react End-labeling

· 100 bp ~ 1 Kb labeled probe DNA purification etc

DNA-spin™ Plasmid DNA Purification Kit

 ● Description

Spin Column type product that design for isolate or purify, over 20ug high purity Plasmid DNA

· High reproducibility of over 20ug high yield and purified plasmid DNA

· Suitable isolation and purification for over 30kb

· Lysis & Neutralization indicator (Lysis Viewer) applied   

 DNA-Spin™ Plasmid DNA Purification Kit has optimized alkali lysis buffering system and cilica membrane

 to isolate high purifyplamid DNA from bacteria. Neutralization buffering system applied for neutral pH and binding.

 It makes not only stable isolation and purification but also efficient to remove chromosomal DNA, cell debris of

 plasmid DNA for over 30 Kb. Lysis Viewer is applied to visual pH confirmation as lysis & neutralization indicator,

 so you can easily find the pH condition during the alkaline lysis step (Fig 1.). High-copy plasmid DNA and low

-copy plasmid DNA are all available to use. Washing Buffer A is separately offered for efficient isolation and 

purification of plasmid DNA from endA+ strain(HB101, the JM strains, NM series strains, PR series strains).

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