Protein Synthesis

YLEX Yeast Expression System (Yarrowia lipolytica) 


 F-Detector™ Cell Free Labeling Kit 

● Description Cell free labeling product to extract protein after in vitro protein expression by fluorescein. §  Non-isotope labeling §  Protein analysis at real-time. §  Offering the sensitivity over radioactive isotope using fluorescent imaging instrument. §  No decreasing bioactivity by Labeling protein. §  Direct detection through UV trans-illuminator §  Detection of proper CE by Laser induced fluorescence.    : It is possible to cooperate with Gene function analysis and drug screening and mutation      screening etc. F-DetectorTM Cell Free Labeling Kit serves a sensitive, non-isotopic, fluorescence-based method for the detection of nascent proteins directly in gels with high signal-to noise ratios after in vitro synthesis. A fluorescent technique using F-Detector™ Kit offers a simplified alternative method that dramatically reduces the time required to obtain data due to the rapid detection of the protein bands immediately after electrophoresis without any downstream processing and eliminates manipulations associated with radioactivity( i.e. gel fixing and drying or transferring to a membrane). It also eliminates the requirement for electroblotting associated with other non-isotopic technologies based on the biotin moiety.

Genelator™ in vitro Transcription/Translation Kit ● Description Easy and fast in vitro Transcription/Translation in one tube without cell culture. §  Fast protein production within one hour. §  High yield protein harvest, amount of 300 ~ 500 mg/ml. §  Wide range application from small peptide to protein. §  Simple scale-up or scale-down. §  Easy target protein isolation through Western blotting, fluorescent gel scanning or     autoradiography.     (Non-radioactive labeling system: F-Detector) §  Suitable for aggregation-prone protein production, resulting from Eukaryote. §  Various gene expression in one tube. §  T7 RNA polymerase added in advance.   Excellent solubility of protein for Origin of Eukaryote gene expression. (active protein production) The GenelatorTM in vitro Transcription/Translation Kit uses highly productive E. coli lysates, which contain all transcriptional and translational machinery components (chaperones, ribosomes, ribosomal factors, tRNAs, aminoacyl-tRNA synthesis, etc.) as well as T7 RNA polymerase. It is a coupled transcription- translation system that can be used to express full-length proteins from T7 or E.coli promoters in a single-step reaction using supercoiled (plasmid) DNA templates. Using the Genelator™ Kit, up to 600ug/ml biologically active protein can be synthesized within 60minutes. The synthesis reaction can be easily scaled up (to produce mg amount of protein synthesized increases linearly with increased reaction volume).  The GenelatorTM in vitro Transcription/Translation Kit  is more suitable for the enhanced soluble production of aggregation-prone protein compared to conventional cell-free protein synthesis. Genelator™ Kit is prepared from genetically engineered E.coli strains which are modified to have enhanced levels of folding-related factors as a folding aid for better solubility of proteins. Genelator™ Kit serves as a powerful technology for the production of aggregation-sensitive proteins originated from eukaryotes.  We expect this Genelator™ Kit will be helpful in production of a higher level of soluble proteins for further biochemical analysis and proteomics research such as structural genomics and functional genomics. And it is applicable to preparation of protein samples required in the actively growing research areas such as structural genomics, functional genomics, protein chip, diagnosis, protein engineering, bioelectronics including biosensor, and lab-on-a-chip.